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| Rodrigues,José Ariévilo Gurgel; Vanderlei,Edfranck de Sousa Oliveira; Bessa,Érika Façanha; Magalhães,Francisco de Araújo; Paula,Regina Célia Monteiro de; Lima,Vilma; Benevides,Norma Maria Barros. |
| The aim of this study was to evaluate certain molecular characteristics of a sulfated polysaccharide (SPs) with anticoagulant properties, isolated from Caulerpa cupressoides (Chlorophyta). Crude SPs were extracted by proteolytic digestion (papain), followed by ion-exchange chromatography on a DEAE-cellulose column. The fractions obtained were analyzed for molecular mass, 0.5% agarose gel electrophoresis and chemical composition. The activated partial thromboplastin time (APTT) test was applied using normal human plasma and standard heparin (HEP) (193 IU mg-1). The yield was ~ 3%, and the chromatography procedure separated the material into three different SP fractions (F I, F II and F III), eluted at the concentrations of 0.50, 0.75 and 1.00 M of NaCl,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Marine algae; Sulfated macromolecules; Chemical analysis; APTT test. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400007 |
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| Amorim,Rodrigo das Neves dos Santos; Rodrigues,José Ariévilo Gurgel; Holanda,Márjory Lima; Quinderé,Ana Luíza Gomes; Paula,Regina Célia Monteiro de; Melo,Vânia Maria Maciel; Benevides,Norma Maria Barros. |
| The aim of this study was to determine the yield, chemical composition, specific rotation (SR), infrared (IR) spectroscopy and the effect on bacterial growth of a crude sulfated polysaccharide (SP) from the red marine alga G. ornata (Go). Go-1 (25°C), Go-2 (80°C), and Go-3 (80°C) were sequentially extracted and yielded 9.2%. The contents of sulfate (5.88-10.3%) and proteins (0.1-3.7%) were small. The values of SR were [µ]D20°f -19.0, -51.0, and -56.5, respectively. IR spectrums showed the presence of galactose-4 sulfate and absence of 3,6-anydrogalactose-2 sulfate, galactose-6 sulfate and galactose-2 sulfate. SR and IR techniques confirmed SPs. Go-3 was tested on the growth of bacteria (Bacillus subtilis, Staphylococcus aureus, Enterobacter aerogens,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rhodophyta; Sulfated macromolecules; Chemical analysis; Antimicrobial. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200001 |
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| Rodrigues, José Ariévilo Gurgel; REDE NORDESTE DE BIOTECNOLOGIA; Vanderlei, Edfranck de Sousa Oliveira; UNIVERSIDADE FEDERAL DO CEARÁ; Quinderé, Ana Luíza Gomes; UNIVERSIDADE FEDERAL DO CEARÁ; Fontes, Bruno Pedrosa; UNIVERSIDADE FEDERAL DO CEARÁ; Benevides, Norma Maria Barros; UNIVERSIDADE FEDERAL DO CEARÁ. |
| A incidência de doenças cardiovasculares e os efeitos adversos da heparinoterapia têm motivado a busca por novos agentes terapêuticos e os polissacarídeos sulfatados (PS) de algas marinhas têm sido reportados como fontes alternativas para tal. Objetivou-se avaliar o potencial anticoagulante dos PS totais (PST) isolados e fracionados das clorofíceas Caulerpa racemosa e Caulerpa cupressoides. Inicialmente, os PST foram extraídos com papaína em tampão acetato de sódio 0,1 M (pH 5,0) contendo cisteína 5 mM e EDTA 5 mM, seguidos de fracionamento em coluna de troca iônica de DEAE-celulose com gradiente de NaCl. As frações obtidas foram analisadas por eletroforese em gel de agarose a 0,5% e a atividade anticoagulante, mensurada pelo tempo de tromboplastina... |
| Tipo: Bioprospecção |
Palavras-chave: 2.08.00.00-2 algas verdes; Macromoléculas sulfatadas; Coagulação sanguínea; TTPA Química de macromoléculas green algae; Sulfated macromolecules; Blood coagulation; APTT. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5923 |
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